Changes in the Phospholipid Asymmetry of Buffalo Sperm Plasma Membrane in Relation to Cryopreservation Technology
PAKISTAN VETERINARY JOURNAL
Authors: Ivanova, M. G.; Gradinarska, D. G.; Genov, M. S.; Tsvetkov, T. S.; Georgiev, B. A.
Abstract
This study evaluates the integrity of the plasma membrane (PM), and the mitochondrial transmembrane potential and their relationship with the motility and kinematic parameters of buffalo spermatozoa frozen in pellets and straws. Ejaculates (n=20) from eight Bulgarian Murrah buffaloes were frozen in pellets (Nagaze-Niva; medium GH22L (R)) and in straws (Cassou; medium Triladyl (R)) at final sperm concentration 80 x 10(6)/ml. The comparative CASA analysis of the motility and kinematic parameters of spermatozoa showed significant differences in the rapid motility and in the VCL values in favor of pellets vs straws (P<0.05). The molecular changes in PM phospholipid asymmetry (assessed by double staining Annexin V-Cy3 (TM) Apoptosis Detection Kit) were higher after freezing - thawing, when compared to the fresh semen (P<0.05). No significant differences were observed in the number of live and functional spermatozoa with intact PM (6CF+/Ann V Cy3-) and the number of apoptotic spermatozoa (6CF+/Ann V Cy3+) between the two freezing technologies. The percentage of spermatozoa with well preserved and functioning mitochondria visualized by Rhodamine123 (Rh123) in the fresh semen was higher, when compared to spermatozoa after cryopreservation (P<0.05). An increase in the percentage of cells with nonfunctional mitochondria (Rh123- or Rh123+) was observed after cryopreservation using both freezing technologies. In conclusion, the freezing of buffalo sperm in pellets, using GH22L (R) protective medium, preserves high sperm motility, kinematics, PM integrity, and mitochondrial transmembrane potential, which is a guarantee of good fertilization capacity of the spermatozoa. (C) 2018 PVJ. All rights reserved
Tunable V-Cavity Laser based Transmitter Optical Sub-Assembly with a Wavelength Locker
2016 15TH INTERNATIONAL CONFERENCE ON OPTICAL COMMUNICATIONS AND NETWORKS (ICOCN)
Authors: Cui, Xin; Qin, Bowen; Li, Da; Cheng, Xiangdong; Jin, Hushan; Xiong, Xiaohai; He, Jian-Jun
Abstract
A wavelength locker is designed and fabricated for a V-cavity laser (VCL) based transmitter optical sub-assembly (TOSA). The tunable VCL and the wavelength locker are packaged together into a small-form-factor 20-pin TOSA box, which is only about 4.4 x 9.5mm(2), and an electronic driver is developed for wavelength tuning and locker control. The frequency of the laser is stabilized within perpendicular to 0.1GHz, and the response time of the locker control is about 4s.
COA
Publication 
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