Total PSA (Human) ELISA Kit

Name: Total PSA (Human) ELISA Kit
SKU: DEIA2339
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Human serum, plasma, urine and tissue culture media

Intended Use

The Total PSA (human) ELISA kit is a complete kit for the quantitative determination of total PSA in human serum, plasma, urine and tissue culture media.

Storage

All components of this kit should be stored at 4°C until expiration.

Sensitivity

The sensitivity or limit of detection of this assay is 0.287pM (0.024ng/mL according to the formulation of PSA WHO International
Standard). The assay sensitivity was determined by interpolation at two standard deviations above the net OD of 20 zero standard replicates utilizing a four parameter logistic (4PL) curve fit. The sensitivity or limit of detection of this assay is 0.287pM
(0.024ng/mL according to the formulation of PSA WHO International Standard). The assay sensitivity was determined by interpolation at two standard deviations above the net OD of 20 zero standard replicates utilizing a four parameter logistic (4PL) curve fit.

General Description

Prostate-specific antigen (PSA), also known as gammaseminoprotein or kallikrein-3 (KLK3), is a kallikrein-like protease produced by the epithelial cells in the prostate gland, the lining of the urethra, and the bulbourethral gland1. It is a single-chain glycoprotein with a molecular weight of 30-33 kDa in human seminal plasma and it functions normally in the liquefaction of seminal coagulum2-3. Under normal conditions, very little PSA is secreted in the blood. PSA exists in serum in multiple forms. The proteolytic activity of PSA in blood is inhibited by irreversible formation of complexes with proteinase inhibitors such as alpha-1-antichymotrypsin (PSA-ACT complex), alpha-2-macroglobulin and other acute phase proteins1-3. A small amount of PSA is not protein bound and is called "free PSA", which is proteolytically inactive4. Increases in glandular size and tissue damage caused by benign prostatic hypertrophy, prostatitis, or prostate cancer may increase circulating total PSA levels5. Monitoring the total PSA level in male blood has been used as an indicator for abnormal prostate function in the past decades. This assay provides an equal molar detection on different forms of PSA in human matrices, which improves the accuracy and sensitivity of the measurement on the total PSA levels6-7.

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Documents

pdf COA

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pdf Datasheet

QA & Reviews

Customer Reviews

References

Association of long-chain non-coding RNAGAS5gene polymorphisms with prostate cancer risk and prognosis in Chinese Han population

MEDICINE

Authors: Zhao, Lisha; Zheng, Weihong; Li, Chen

Abstract

Background: To investigate the correlation between growth arrest-specific transcript 5 (GAS5) gene polymorphism and the risk and prognosis of prostate cancer in Chinese Han population. Methods: Sanger sequencing was used to analyze genotypes at the rs17359906 and rs1951625 loci of theGAS5gene in 218 prostate cancer patients and 220 healthy controls. The follow-up period was from August 2016 to August 2019, and the relationships betweenGAS5gene polymorphisms at the rs17359906 and rs1951625 loci and the recurrence-free survival rate of prostate cancer patients were analyzed. Results: GAS5A-allele carriers at the rs17359906 locus were 3.44 times more likely to develop prostate cancer than G-allele carriers (95% confidence interval (CI): 2.38-4.96,P < .001). Carriers of theGAS5A allele at the rs1951625 locus had a 1.40-fold higher risk of prostate cancer than carriers of the G allele (95% CI: 1.05-1.86,P = .027). Plasma prostate-specific antigen (PSA), body mass index (BMI), and rs17359906 and rs1951625 loci were independent risk factors for prostate cancer.GAS5AA genotype and A-allele carriers (GA + AA) at the rs1951625 locus were significantly correlated with Gleason scores <= 7 (P < .05).GAS5genes rs17359906 G > A and rs1951625 G > A were associated with high plasma PSA levels. The recurrence-free survival rate of patients with prostate cancer with AA genotype at the rs17359906 locus ofGAS5(66.67%) was significantly lower than that of the GA genotype (76.47%), whereas the GG genotype was the highest (91.96%), and the difference was statistically significant (P = .002). The recurrence-free survival rate of patients with prostate cancer with the AA genotype at the rs1951625 locus ofGAS5(75.00%) was significantly lower than that of the GA genotype (81.82%), whereas the GG genotype was the highest (87.76%) with a statistically significant difference (P = .025). Conclusion: GAS5rs17359906 G > A and rs1951625 G > A are significantly associated with an increased risk of prostate cancer and a reduction in three-year relapse-free survival.

Empirical analysis of partial discharge data and innovative visualization tools for defect identification under DC stress

INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS

Authors: Madhar, Saliha Abdul; Mraz, Petr; Mor, Armando Rodrigo; Ross, Rob

Abstract

This paper presents several approaches to the analysis of partial discharge (PD) data. Three common defects namely corona, surface and floating electrode are studied with the goal of defect identification under DC stress conditions. One of the major concerns with DC-PD testing, is its non-repetitive/erratic pulse pattern. This paper, however, only deals with the repetitive stages of discharge that will allow the study of their resultant patterns and trends. Several unique features such as the formative trend in the probability plot of time between discharges for the three common defect types shows promise in the quest for defect identification under DC. Further, the paper also describes in which way a three-pulse PSA diagram cannot serve as a standalone figure and hence requires a change in perspective by either adding or reducing a dimension. The last part of the paper presents a test methodology to identify the discharge source based on various discharge features.