Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene
Nature
Recombinant
Tag/Conjugate
Unconjugated
Purity
> 99% , as determined by SDS-PAGE
Format
Liquid
Concentration
50,000 U/mL
Buffer
50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9
Storage
Store at -20°C
Introduction
T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.
Keywords
T7; RNA Polymerase; T7 promoter
Citations
Publication ()
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