T7 RNA Polymerase ELISA Kit

Sample

Universal

Species Reactivity

NA

Detection Method

sELISA

Intended Use

It is suitable for the determination of T7 RNA Polymerase content in the samples.

Storage

Anti-T7 RNA Polymerase, Streptavidin HRP, T7 RNA Polymerase Standard: -18°C
Others: 2-8°C, Store away from light.
Shelf life: 12 months

Precision

CV%≤10%,RE%≤±15%

Detection Limit

Detection range: 0.25 ~ 16 ng/mL
Limit of quantitation: 0.25 ng/mL
Limit of detection: 0.012 ng/mL

General Description

T7 RNA Polymerase (T7 RNA Polymerase) is a protein encoded by phage T7 DNA expressed by recombinant E.coli. It is a DNA-dependent 5'→3' RNA polymerase that highly specifically recognizes the T7 promoter sequence. T7 RNA polymerase can use single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and use NTP as a substrate to synthesize RNA complementary to the single-stranded DNA or double-stranded DNA template strand downstream of the promoter.

Standard Curve