Synthetic Cannabinoids (JWH-250) ELISA Kit

Name: Synthetic Cannabinoids (JWH-250) ELISA Kit
SKU: DEIA-XYZ229
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, Blood, Serum,

Species Reactivity

Human; Research Animal

Detection Method

cELISA

Intended Use

For the determination of trace quantities of synthetic cannabinoids and metabolites in human blood, serum, and urine.

Contents of Kit

1. EIA Buffer: 30 mL (ready to use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of samples.
2. Wash Buffer Concentrate (10×): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. Substrate: 20 mL (ready to use). Stabilized 3,3',5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light sensitive.
4. Drug-Enzyme Conjugate: 14 mL (ready to use). Drug-horseradish peroxidase conjugate. Do Not Dilute.
5. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is.
6. Acid Stop Solution: 14 mL (ready to use). 1N H₂SO₄ used to stop the enzyme reaction.
7. Qualitative QC Positive Control: 750 μL provided (JWH-018 pentanoic acid in synthetic human urine). Do not dilute.
8. Qualitative QC Negative Control: 750 μL provided (synthetic human urine). Do not dilute.

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C.

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

Creative Diagnostics's Synthetic Cannabinoids (JWH-250) RTU ELISA Kit is a qualitative one-step kit designed for use as a screening device for the detection of JWH-250, JWH-018, JWH-073, JWH-200, JWH-203, RCS-8, AM2201, and other synthetic cannabinoid metabolites. The kit was designed for screening purposes and is intended for research use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Standard Curve