Anti-JWH-250 polyclonal antibody

A LC-HRMS (liquid chromatography coupled with high resolution mass spectrometry) method for the detection and quantitation of several synthetic cannabinoids (JWH-018, JWH-019, JWH-073, JWH-081, JWH-122 JWH-200, JWH-250, AM-2201, (+/-)-CP 47,497, (C8)-CP 47,497, HU-211) and selected metabolites (JWH-018 N-(4-hydroxypentyl) and JWH-073 N-(3-hydroxybutyl)) in whole blood and urine was developed and validated. These methods were applied to postmortem cases from the Johannesburg Forensic Pathology Services Medicolegal Laboratory (FPS-MLL) to assess the prevalence of these synthetic cannabinoids amongst the local postmortem population. Urine samples were extracted utilizing a solid phase extraction (SPE) method, while blood samples were extracted utilizing liquid-liquid extraction (LLE) method. The accuracy of the analytes ranged from 88-107 % in whole blood (relative standard deviation (RSD) = 7.5-15.0 %), and from 95-109 % in urine (RSD = 4.9-11.9 %). The limits of detection (LOD) and the limits of quantitation (LOQ) for the analytes ranged between 0.675 ng mL(-1) and 3.375 ng whole blood, and between 0.225 ng mL(-1) to 3.375 ng mL(-1) in urine. None of the postmortem cases tested positive for any of the targeted analytes. The sample population could be extended to living subjects such as those in drug rehabilitation centres or in hospitals to obtain a more accurate representation of the overall usage in South Africa.

Purpose.- Synthetic cannabinoids (SCs) are the largest group of new psychoactive substances (NPS) monitored worldwide. We validate a rapid and sensitive liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assay for the determination of 16 SCs in human hair samples. This method was successfully applied to document chronic use of JWH-122 in a 42 years-old male included in large-scale cross-sectional study in Paris. Method.- The SCs JWH-018, JWH-018-5-N(OH-pentyl), JWH-073, JWH-073-4-N(OH-butyl), JWH-122, JWH-200, JWH-019, JWH-250, AM-2201, EAM-2201, BB-22, ADB-PINACA, AKB48-5F, AB-FUBINACA, 5F-AB-PINACA, 5F-PB-22 were extracted from 20 mg of decontaminated, washed and grinded hair by a mixture of hexane/ethyl acetate (1/1, v/v) in alkaline condition in the presence of JWH-018-D11 internal standard. SCs were then separated by a gradient of formate buffer 2 mM in 0.1% formic acid (A) and acetonitrile (B) on Hypersyl Gold PFP column (100 x 2.1 mm i.d., particle size 1.9 mu m, ThermoFisher) at 30 degrees C. The detection was performed on TSQ Vantage triple-quadrupole mass spectrometer (Thermofisher, Les Ulis, France) in positive ionization and multi-reaction monitoring (MRM) modes. A lock of hair (6 cm) was sampled from a patient hospitalized for JWH 122 overdose and segmented into 3 segments: A: 0-2 cm (proximal), B: 2-4 cm and C: 4-6 cm. It was then analyzed with the present method in order to determine past use of synthetic cannabinoids. Results.- The method exhibits a good linearity (r(2) > 0.996) over the range 1-25 to 1000 pg/mg, Limits of detection and quantification ranged from 0.3 to 8 pg/mg and from 1 to 25 pg/mg, respectively (CVs <= 15%). No interference from endogenous substances with the detection of SCs and their IS was observed and the carryover was insignificant. Intraday and inter-day trueness (bias) and precision (CVs) were <= 8.2%, <= 14.3%, <= 8.3% and <= 14.9%, respectively. Overall recoveries ranged between 61 and 94% and corrected matrix effect from 31 to 110%. JWH-122 hair concentrations found in patient's hair were: (A/B/C: 200/450/430 pg/mg) in agreement with the concentrations found in the literature suggesting chronic use of JWH-122 over the last six months. Conclusion.- The present LC-MS/MS method proved to be suitable for the detection and quantification of 16 synthetic cannabinoids in human hair. (C) 2019 Societe Francaise de Toxicologie Analytique. Published by Elsevier Masson SAS. All rights reserved.