Recombinant HIV Reverse Transcriptase

Product Overview

Solution.

Nature

Recombinant

Tag/Conjugate

Unconjugated

Format

Liquid

Buffer

In 10 mM potassium phosphate, pH 7.4, 1 mM DTT and 20% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

The HIV reverse transcriptase has ribonuclease activity that degrades the viral RNA during the synthesis of cDNA, as well as DNA-dependent DNA polymerase activity that copies the sense cDNA strand into an antisense DNA to form a double-stranded viral DNA intermediate (vDNA).

Antigen Description

HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.