Recombinant HIV-2 Reverse Transcriptase

Name: Recombinant HIV-2 Reverse Transcriptase
SKU: DAG-P2933
Rating: 5 (1 reviews)

HIV-2 Reverse Transcriptase, recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Nature

Recombinant

Tag/Conjugate

TBD

Purity

≥85% as determined by SDS-PAGE

Format

Liquid or Lyophilized powder

Buffer

Tris/PBS-based buffer, 5%-50% glycerol

Preservative

None

Storage

Store at -20°C to -80°C

Introduction

The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic

Keywords

p66 RT; Reverse transcriptase/ribonuclease H; HIV2 RT

Citations

Have you cited DAG-P2933 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Lack of Evidence of Simian Immunodeficiency Virus Infection Among Nonhuman Primates in Tai National Park, Cote d'Ivoire: Limitations of Noninvasive Methods and SIV Diagnostic Tools for Studies of Primate Retroviruses

INTERNATIONAL JOURNAL OF PRIMATOLOGY

Authors: Locatelli, Sabrina; Roeder, Amy D.; Bruford, Michael W.; Noe, Ronald; Delaporte, Eric; Peeters, Martine

Abstract

It is now well established that the human immunodeficiency viruses, HIV-1 and HIV-2, are the results of cross-species transmissions of simian immunodeficiency viruses (SIV) naturally infecting nonhuman primates in sub-Saharan Africa. SIVs are found in many African primates, and humans continue to be exposed to these viruses by hunting and handling primate bushmeat. Sooty mangabeys (Cercocebus atys) and western red colobus (Piliocolobus badius badius) are infected with SIV at a high rate in the Tai Forest, Cote d'Ivoire. We investigated the SIV infection and prevalence in 6 other monkey species living in the Tai Forest using noninvasive methods. We collected 127 fecal samples from 2 colobus species (Colobus polykomos and Procolobus verus) and 4 guenon species (C. diana, C. campbelli, C. petaurista, and C. nictitans). We tested these samples for HIV cross-reactive antibodies and performed reverse transcriptase-polymerase chain reactions (RT-PCR) targeting the gag, pol, and env regions of the SIV genome. We screened 16 human microsatellites for use in individual discrimination and identified 4-6 informative markers per species. Serological analysis of 112 samples yielded negative (n=86) or uninterpretable (n=26) results. PCR analysis on 74 samples confirmed the negative results. These results may reflect either the limited number of individuals sampled or a low prevalence of infection. Further research is needed to improve the sensitivity of noninvasive methods for SIV detection.

Synthesis and in vitro anti-HIV evaluation of a new series of 6-arylmethyl-substituted S-DABOs as potential non-nucleoside HIV-1 reverse transcriptase inhibitors

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Authors: Wang, Yue-Ping; Chen, Fen-Er; De Clercq, Erik; Balzarini, Jan; Pannecouque, Christophe

Abstract

A series of new 5-alkyl-2-benzylsulfanylpyrimidin-4(3H)-ones (5a-y) bearing different substituted arylmethyl moieties at the C-6 position of the pyrimidine core have been synthesized and evaluated for their in vitro activities against HIV-1 and HIV-2 in MT-4 cell cultures. The majority of the title compounds showed moderate to good activities against HIV-1 with an IC50 range from 6.67 mu M to 0.12 mu M. Among them, 6-(3,5-dimethylbenzy]) analogue 5q exhibited the most potent anti-HIV-1 activity (IC50 = 0.12 mu M, SI > 2642), which was about 40-fold more active than the reference compounds 1-[(2-hydroxyethoxy)methyl]-6-(phenylsulfanyl)thymine (HEPT) and 2',3'-dideoxyinosine (DDI). The structure-activity relationships (SARs) of these new congeners were further discussed. (C) 2008 Elsevier Masson SAS. All rights reserved.