10 mM Phosphate Buffered Saline, pH 7.2 with 3M Urea
Preservative
None
Storage
Short Term (≤ 1 week): 2-8°C. Long Term: -20°C. Avoid repeated freezing and thawing.
Introduction
Coronaviruses are a diverse group of single-stranded plus-sense RNA viruses belonging to the Coronaviridae family and Nidovirales order. They infect a wide range of mammalian and avian species, including bats, and can jump into new species and be transmitted zoonotically as shown by severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). Coronaviruses contain an envelope, for membrane fusion with host cell membranes and this is a critical step in the replication cycle allowing for delivery of genomic RNA into the cytoplasm, for replication. This pathway is therefore an attractive target for therapeutic interventions.
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