This test kit allows rapid and quantitative measurement of rat anti-SRBC IgM levels in serum or plasma samples.
Contents of Kit
1. SRBC Coated 96-well Plate (provided as 12 strips of 8 wells) 2. Enzyme Conjugate Reagent, 11 ml 3. Reference Standard Stock (lyophilized) 4. 20× Wash Solution: 50 ml 5. Diluent: 30 ml 6. TMB Reagent (One-Step): 11 ml 7. Stop Solution (1N HCl): 11 ml
Storage
The kit should be stored at 4°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.
General Description
Recent studies have demonstrated that suppression of anti-SRBC (sheep red blood cell) IgM levels by therapeutic agents serves as a useful indicator of immunosupression.1,2 This test kit allows rapid and quantitative measurement of rat anti-SRBC IgM levels in serum or plasma samples.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y-axis against anti-SRBC IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.
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