Species Reactivity
Chicken
Intended Use
This test kit allows rapid and quantitative measurement of chicken anti-SRBC IgM levels in serum or plasma.
Storage
The kit should be stored at 2-8°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.
General Description
Measurement of anti-SRBC (sheep red blood cell) immunoglobulin levels is used to assess immune function in chickens. To date, a somewhat qualitative hemagglutination assay has often been used to measure antibody titers. Disadvantageously, the hemagglutination assay does not readily differentiate between IgM and IgG (IgY) responses. To address these issues, we at Creative Diagnostics, Inc. have developed an enzyme linked immunosorbant assay (ELISA) that allows rapid and quantitative measurement of chicken anti-SRBC IgM levels in serum or plasma.
Citations
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Smith, CC; Nelson, J; et al. Ras-GAP binding and phosphorylation by herpes simplex virus type 2 RR1 PK (ICP10) and activation of the Ras/MEK/MAPK mitogenic pathway are required for timely onset of virus growth. JOURNAL OF VIROLOGY 74:10417-10429(2000).
MAO, SY; YAMASHITA, T; et al. CHEMICAL CROSS-LINKING OF IGE-RECEPTOR COMPLEXES IN RBL-2H3 CELLS. BIOCHEMISTRY 34:1968-1977(1995).
COA
Certificate of Analysis
