Chicken SRBC IgG ELISA Kit

Sample

Serum, plasma

Species Reactivity

Chicken

Detection Method

sELISA

Intended Use

ELISA for the quantitative determination of chicken anti-sheep red blood cell (SRBC) IgG in serum and plasma.

Contents of Kit

1.SRBC coated 96-well plate, 96 (12 x 8) wells
2.Enzyme Conjugate Reagent, 11 mL
3.Reference standard stock (lyophilized), 1 vial
4.20x Wash Solution, 50 mL
5.Diluent, 50 mL
6.TMB Reagent (One-Step), 11 mL
7.Stop Solution (1N HCl), 11 mL
Note: Chicken anti-SRBC IgG levels are measured in nominal units and are calibrated with reference to chicken anti-SRBC serum.

Storage

The kit should be stored at 2-8°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.

Detection Range

6.25 to 100 u/mL

General Description

Measurement of anti-SRBC (sheep red blood cell) immunoglobulin levels is used to assess immune function in chickens. To date, a somewhat qualitative hemagglutination assay has often been used to measure antibody titers. Disadvantageously, the hemagglutination assay does not readily differentiate between IgG (IgY) and IgM responses. To address these issues, we have developed an enzyme linked immunosorbent assay (ELISA) that allows rapid and quantitative measurement of chicken anti-SRBC IgG levels in serum or plasma.
Studies have demonstrated that IV immunization of chickens with 0.5 mL of a 10% solution of SRBC caused an elevation of anti-SRBC IgG levels from undetectable levels on day zero to ~11,000 units/mL on day seven.

Standard Curve

A typical standard curve with optical density readings at 450 nm on the Y axis against anti-SRBC IgG concentrations on the X axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.