Rat NGAL ELISA Kit

Sample

urine, plasma or serum

Species Reactivity

Rat

Detection Method

sELISA

Intended Use

For the in-vitro determination of rat NGAL in urine, plasma or serum. For research use only.

Storage

1. Store the kit with all reagents at 2-8°C. Do not freeze.
2. Use all reagents before the expiry date on the kit box label.

Precision

Intraassay variation was determined by measurement of rat NGAL in two urine samples and two EDTA plasma samples with 8 replicates. The following results were obtained (CV = coefficient of variation):

Interassay variation was determined by measuring rat NGAL in six diluted urine samples and five diluted EDTA plasma samples with 2 replicates in 2-4 separate assays. The following results were obtained:

Detection Limit

The lowest concentration of rat NGAL giving an absorbance reading greater than 2SD above the mean zero (Calibrator 1) reading (n = 20) was determined to 0.5 pg/mL. This was significantly lower than the value of Calibrator 2 (4 pg/mL).

General Description

NGAL is a biomarker of kidney tubular damage and a biological response to the damage process which is distinctly separated from loss of kidney function called Acute Kidney Injury (AKI). As damage often precedes loss of function renal expression of NGAL increases dramatically in a variety of causes and NGAL is released into both urine and plasma. Accordingly, renal disorders with presence of acute tubular damage are associated with raised urinary and plasma NGAL levels. Urinary NGAL serves as an early marker of acute kidney injury after cardiopulmonary bypass surgery and both urinary and plasma NGAL levels provide an early indication of acute renal injury in unselected patients in intensive care4. Raised urinary and plasma levels of NGAL have also been observed in patients with chronic kidney diseases, and persistently raised urinary levels after renal transplantation are associated with poor graft function.