Human NGAL ELISA Kit

Sample

urine and EDTA plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

An enzyme-linked immunosorbent assay for quantitative determination of human NGAL in urine and EDTA plasma. For Research Use Only.

Storage

1. Store the kit with all reagents at 2-8°C. Do not freeze.
2. Use all unopened reagents before the expiry date on the kit box label.
3. The stability of materials/reagents after opening is indicated on the kit box label.
4. Diluted Wash Solution Concentrate remains stable for 4 weeks at 2-8°C. If not using all wells, dilute only the portion of Wash Solution Concentrate required.
5. Diluted Sample Diluent Concentrate remains stable for 4 weeks at 2-8°C. If not using all wells, dilute only the portion of Sample Diluent Concentrate required.
6. For subsequent use, store unused wells in the foil pouch with the desiccant provided and reseal. Always allow the foil pouch to equilibrate to room temperature before opening to avoid condensation in/on the coated microwells.

Performance Characteristics

TRACEABILITY OF CALIBRATOR VALUE
No internationally approved reference material for NGAL is currently available.The NGAL concentration of calibrator material has been assigned by turbidimetry using a precise transfer protocol ensuring traceability to the CD master calibrator.The reference material was value-assigned by measurement of light absorbance at 280 nm using a theoretically calculatedextinction coefficient based on the amino-acid composition.

General Description

NGAL is a biomarker of kidney tubular damage and a biological response to the damage process which is distinctly separated from loss of kidney function called Acute Kidney Injury (AKI). As damage often precedes loss of function renal expression of NGAL increases dramatically in a variety of causes and NGAL is released into both urine and plasma. Accordingly, renal disorders with presence of acute tubular damage are associated with raised urinary and plasma NGAL levels. Urinary NGAL serves as an early marker of acute kidney injury after cardiopulmonary bypass surgery and both urinary and plasma NGAL levels provide an early indication of acute renal injury in unselected patients in intensive care4. Raised urinary and plasma levels of NGAL have also been observed in patients with chronic kidney diseases, and persistently raised urinary levels after renal transplantation are associated with poor graft function.