Purified Influenza A/PR/8/34 (H1N1)

Product Overview

SPF chicken embryos are inoculated with H1N1 influenza virus. After incubation, the infected allantoic fluid is harvested, tested for sterility, and concentrated by tangential flow filtration. The virus is pelleted by centrifugation, resuspended in HEPES-buffered saline, sonicated, and purified on a sucrose gradient. The purified fraction is washed, resuspended, assayed for total protein, adjusted to 2 mg/mL, and dispensed into 1 mL aliquots.

Target

IAV H1N1

Nature

Native

Format

Liquid

Buffer

HEPES-buffered saline

Storage

Store at -20°C to -80°C. Avoid multiple freeze/thaw cycles.

Introduction

Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.

Keywords

Influenza A virus; IAV; HA; H1N1

Inactivation

β-propiolactone (BPL)