Human H1N1 IgG ELISA Kit (DEIASL351)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Serum, plasma
Species Reactivity
Intended Use
Human Influenza A IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against Influenza A in serum and plasma. The identification aids in the diagnosis of influenza (flu).
The microtiter well plate and all other reagents are stable at 2-8°C until the expiration date printed on the label. The whole kit stability is usually 6 months from the date of shipping under appropriate storage conditions. The unused portions of the standards should be stored at 2-8°C or stored frozen in small aliquots and should be stable for 3 months.
Intra-Assay-Precision 8.5 %
Inter-Assay-Precision 6.5%
Inter-Lot-Precision 3.8-6.1%
Analytical Sensitivity 1.09 U/mL
Clinical Sensitivity 100 %
General Description
The influenza infection is an acute feverish virus infection, which principally leads to an illness of the respiratory tract and appears as an epidemic or pandemic. The infection mostly results from a droplet infection. The virus spreads from the mucous membrane of the upper respiratory to the whole bronchial tract. There the virus and its toxin can lead to a serious inflammation of the bronchial mucosa and a damage of the vessels. After incubation time of 1 to 3 days the symptoms appear suddenly: Followed by a fast increase of temperature, often accompanied by shivering, the catarrhal leading symptom appears, which contribute to the clinical course beside painful dry cough, tracheitis, laryngitis and frequently a rhinitis and conjunctivitis. The Influenza viruses form a virus group with principally similar morphological, chemical and biological features. The types A, B and C were defined, from which many other variants are known. The distinction of the types will be possible by the different antigenicity of their nucleoproteins, which are coated by a matrix protein with type-specific antigenicity, too. However, both internal antigens are of less importance for the immunity. The essential antigens are the hemagglutinin and the neuraminidase. Both are surface antigens and subject to a permanent change of their antigenicity, which is called drift or shift. The appearance of permanent new Influenza epidemics and pandemics are particularly facilitated by an antigen variability, because the new drift or shift variants infect a population which is only partly immune or in an extreme case completely susceptible to the disease. The determination of the Influenza type (A, B, and C) gives both the clinician and epidemiologist important indications for further actions. Thus Influenza B often leads to a serious clinical course and an epidemic spread of the virus. Similarly, during Influenza A epidemic, the epidemiological importance and derived measures for the protection of the individual and population primarily stand in the foreground together with the severity of the clinical symptoms.


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Matsuzaki, Y; Sugawara, K; et al. Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants. JOURNAL OF VIROLOGY 88:12364-12373(2014).
van de Sandt, CE; Kreijtz, JHCM; et al. Human Cytotoxic T Lymphocytes Directed to Seasonal Influenza A Viruses Cross-React with the Newly Emerging H7N9 Virus. JOURNAL OF VIROLOGY 88:1684-1693(2014).

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