This versatile bimodal reporter system integrates both firefly luciferase and mCherry fluorescent protein within the VSV-NiV-G/F pseudotyped framework. The combination of enzymatic luminescence (for quantitative bulk measurements) and red fluorescence (for qualitative cellular visualization) provides comprehensive analytical capabilities in a single reagent. This dual-reporter configuration is particularly valuable for correlating population-level neutralization data with single-cell infection confirmation. Note: 1. Biosafety: These are BSL-2 reagents; they are replication-defective and capable of only single-round infection 2. Storage: -80°C for long-term; avoid repeated freeze-thaw cycles (>3 cycles not recommended) 3. Cell lines: Vero, Vero E6, and PK13 cells are standard targets for NiV-G/F entry studies; verify receptor expression (e attending BSB, Ephrin-B2/B3) 4. Titration: End-users should perform pilot experiments to determine optimal challenge doses for their specific cell models and applications
Store at -80°C. Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80°C refrigerator and melt it in an ice bath.
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