This replication-deficient pseudovirus is generated using a LV-ΔG backbone where the native LV-G glycoprotein is replaced with Nipah virus (NiV) attachment glycoprotein (G) and fusion protein (F). The viral particles incorporate firefly luciferase as a quantitative reporter gene, enabling highly sensitive detection of viral entry through luminescence-based assays. Produced in cells, these pseudoviruses accurately mimic NiV receptor binding and membrane fusion mechanisms while ensuring BSL-2 laboratory safety standards. Note: 1. Biosafety: These are BSL-2 reagents; they are replication-defective and capable of only single-round infection 2. Storage: -80°C for long-term; avoid repeated freeze-thaw cycles (>3 cycles not recommended) 3. Cell lines: Vero, Vero E6, and PK13 cells are standard targets for NiV-G/F entry studies; verify receptor expression (e attending BSB, Ephrin-B2/B3) 4. Titration: End-users should perform pilot experiments to determine optimal challenge doses for their specific cell models and applications
Store at -80°C. Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80°C refrigerator and melt it in an ice bath.
My Review for Pseudotyped LV-NiV G+F Protein-Luciferase
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
