Recombinant S. aureus Protein A (a.a. 37-469)

Product Overview

Recombinant Staphylococcus aureus Protein A (a.a. 37-469)

Nature

Recombinant

Tag/Conjugate

Unconjugated

Purity

> 90% by SDS-PAGE

Format

Liquid. In 20 mM Tris-HC buffer (pH 8.0) containing 10% glycerol, 48.1 kDa (434aa), confirmed by MALDI-TOF.

Buffer

20 mM Tris-HCl, pH 8.0, 10% glycerol

Preservative

None

Storage

Store at -80°C

Introduction

Staphylococcus is a genus of Gram-positive bacteria. Under the microscope they appear round (cocci), and form in grape-like clusters. Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Keywords

S.aureus SpA; Staphylococcal Protein A; Staphylococcal; Protein A; Staphylococcus; Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; Staphylococcus aureus SpA; S. aureus SpA