Bovine PLG(Plasminogen) ELISA Kit

Delayed vascularization and resultant resorption limits the clinical use of tissue engineered bony constructs. The objective of this study is to develop a strategy to accelerate the neovascularization of tissue-engineered bony constructs using endothelial differentiated adipose-derived stem cells (ASC). The authors harvested ASC from inguinal fat pads of male Lewis rats (n = 5) and induced toward endothelial and osteoblastic lineages. The authors created critical size calvarial defects on male Lewis rats (n = 30) and randomized the animals into 4 groups. For the repair of the defects the authors used hydroxyapatite/poly(lactide-co-glycolide) [HA-PLG] scaffolds in group I, HA-PLG scaffolds seeded with ASC in group II, HA-PLG scaffolds seeded with ASC-derived endothelial cells in group III, and HA-PLG scaffolds seeded with ASC-derived osteoblasts in group IV. The authors evaluated the bone healing histologically and with microcomputed tomography (CT) scans 8 weeks later. Adipose-derived stem cells exhibited the characteristics of endothelial and osteogenic lineages, and attached on HA-PLG scaffolds after differentiation. Micro-CT analysis revealed that highest bone mineral density was in group IV (1.46 +/- 0.01 g/cm(3)) followed by groups III (1.43 +/- 0.05 g/cm(3)), I (1.42 +/- 0.05 g/cm(3)), and II (1.3 +/- 0.1 g/cm(3)). Hematoxylin-Eosin and Masson Trichrome staining revealed similar results with the highest bone regeneration in group IV followed by groups II, III, and I. Regenerated bone in group IV also had the highest vascular density, but none of these differences achieved statistical significance (P > 0.05). The ASC-derived endothelial cells and osteoblasts provide a limited increase in calvarial bone healing when combined with HA-PLG scaffolds.

Poplar leaves graphene (PLG) was synthesized from the poplar leaves. Modified poplar leaves graphene (M-PLG) was obtained by graphene treated with silane coupling agent gamma-aminopropyltriethoxysilane (KH550). The M-PLG-modified silicone rubber (methyl ethylene silicon rubber [MVQ]) composites were prepared by mechanical blending. The effects of M-PLG on the mechanical properties, thermal properties, and morphology structure of MVQ were investigated. The results showed that the carbon content and purity of the PLG were high. The M-PLG can be evenly dispersed into MVQ compared with the PLG, the mechanical properties of the M-PLG/MVQ composites were better than those of the PLG/MVQ when M-PLG content was 0.1 parts per hundred rubber (phr), the tensile strength and elongation at break are increased by 36.2% and 19.4% respectively, and wear resistance increased by 57.1%. The thermal stability of the M-PLG/MVQ was also better. This concept of combining PLG with MVQ demonstrated the potential of preparing novel green materials with excellent properties.