Murine Plasminogen Matched Antibody Pair (ABPR-L032)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
Sufficient reagent for 4 x 96 well plates
Sample
Plasma
Species Reactivity
Murine
Intended Use
This antibody pair set comes with matched antibody pair to detect and quantify protein level of Murine PLG
Contents of Kit
1. Capture Antibody (yellow): 0.4 ml of polyclonal affinity-purified anti-Pg antibody for coating plates.
2. Detecting Antibody (red): 0.4 ml of affinity-purified peroxidase conjugated polyclonal anti-Pg antibody for detection of captured Pg.
Note: Reagents are sufficient for at least 4×96 well plates using recommended protocols. Antibodies are supplied in a 50% (v/v) glycerol solution for storage at –10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.
Storage
-10 to -20°C
General Description
Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2 Antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2 Antiplasmin. Other physiological inhibitors of plasmin include α2 macroglobulin and antithrombin.

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