Human Plasminogen Total antigen ELISA Kit

Name: Human Plasminogen Total antigen ELISA Kit
SKU: DEIA9557
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

biological fluids

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Human Plasminogen Total antigen ELISA Kit is used for the quantitative determination of total plasminogen and plasmin in biological fluids.

Contents of Kit

1. 96-well antibody coated microtiter strip plate (removable strips 8 x 12): Containing anti-human plasminogen antibody dried and blocked on the strip well surface
2. 10X Wash Buffer: 1 bottle of 50 mL; bring to 1 x using DI water
3. Human Plasminogen activity Standard: 1 vial lyophilized Standard
4. Anti-plasminogen primary antibody: 1 vial lyophilized polyclonal sheep anti-mouse antibody
5. Anti-sheep horseradish peroxidase secondary antibody: 1 vial concentrated HRP labeled antibody
6. TMB substrate solution: 1 bottle 10 mL solution

Storage

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -70°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Piperlongumine restores the balance of autophagy and apoptosis by increasing BCL2 phosphorylation in rotenone-induced Parkinson disease models

AUTOPHAGY

Authors: Liu, Jia; Liu, Weijin; Lu, Yongquan; Tian, Hao; Duan, Chunli; Lu, Lingling; Gao, Ge; Wu, Xia; Wang, Xiaomin; Yang, Hui

Abstract

Parkinson disease (PD) is the second most common neurodegenerative disorder after Alzheimer disease and is caused by genetics, environmental factors and aging, with few treatments currently available. Apoptosis and macroautophagy/autophagy play critical roles in PD pathogenesis; as such, modulating their balance is a potential treatment strategy. BCL2 (B cell leukemia/lymphoma 2) is a key molecule regulating this balance. Piperlongumine (PLG) is an alkaloid extracted from Piper longum L. that has antiinflammatory and anticancer effects. The present study investigated the protective effects of PLG in rotenone-induced PD cell and mouse models. We found that PLG administration (2 and 4 mg/kg) for 4 wk attenuated motor deficits in mice and prevented the loss of dopaminergic neurons in the substantia nigra induced by oral administration of rotenone (10 mg/kg) for 6 wk. PLG improved cell viability and enhanced mitochondrial function in primary neurons and SK-N-SH cells. These protective effects were exerted via inhibition of apoptosis and induction of autophagy through enhancement of BCL2 phosphorylation at Ser70. These results demonstrate that PLG exerts therapeutic effects in a rotenone-induced PD models by restoring the balance between apoptosis and autophagy.

Cell proliferation influenced by matrix compliance of gelatin grafted poly(D,L-Lactide) three dimensional scaffolds

COLLOIDS AND SURFACES B-BIOINTERFACES

Authors: Balavigneswaran, Chelladurai Karthikeyan; Mahto, Sanjeev Kumar; Mahanta, Arun Kumar; Singh, Rajshree; Vijayakumar, Mahalingam Rajamanickam; Ray, Biswajit; Misra, Nira

Abstract

Surface and mechanical properties of the biomaterials are determinants of cellular responses. In our previous study, star-shaped poly(D,L-Lactide)-b-gelatin (ss-pLG) was reported for possessing improved cellular adhesion and proliferation. Here, we extended our investigation to establish the cellular compatibility of gelatin-grafted PDLLA with respect to mechanical properties of biological tissues. In this view, linear PDLLA-b-gelatin (l-pLG) was synthesized and tissue-level compatibility of 1-pLG and ss-pLG against fibroblasts (L929), myoblasts (C2C12) and preosteoblasts (MG-63) was examined. The cell proliferation of C2C12 was significantly higher within 1-pLG scaffolds, whereas L929 showed intensified growth within ss-pLG scaffolds. The difference in cell proliferation may be attributed to the varying mechanical properties of scaffolds; where the stiffness of 1-pLG scaffolds was notably higher than ss-pLG scaffolds, most likely due to the variable levels of gelatin grafting on the backbone of PDLLA. Therefore, gelatin grafting can be used to modulate mechanical property of the scaffolds and this study reveals the significance of the matrix stiffness to produce the successful 3D scaffolds for tissue engineering applications. (C) 2018 Elsevier B.V. All rights reserved.