Penicillin G [BSA]

Product Overview

Penicillin G, BSA-conjugate

Target

PCN

Nature

Synthetic

Tag/Conjugate

BSA

Format

Liquid

Concentration

2.0 mg/ml BSA

Buffer

PBS buffer

Preservative

None

Storage

Store at -20°C. Avoid repeated freeze/thaw cycles

Introduction

Penicillin is a group of Beta-lactam antibiotics used in the treatment of bacterial infections caused by susceptible, usually Gram-positive, organisms. beta-lactam antibiotics work by inhibiting the formation of peptidoglycan cross-links in the bacterial cell wall, which results in cytolysis.

Antigen Description

The penicillin G (benzylpenicillin) and BSA (bovine serum albumin) (10 mg each) are conjugated by EDC method in 0.1 M MES pH 5.0. The carboxyl group in the penicillin G is directly linked to an amine group in the BSA without any linker by EDC conjugation method. Given the molecular weights of penicillin G and BSA are 334. 4 Da and 66.4 kDa, respectively, the molar ratio of penicillin G:BSA in the conjugation solution is 199:1. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS, pH 7.4. The number of penicillin G that is actually conjugated to each BSA molecule is not determined.

Keywords

Ampicillin; Benzylpenicillin; PCN; Penicillin G; Phenoxymethylpenicillin; Penicillin