Neutral Protease Assay Kit

Name: Neutral Protease Assay Kit
SKU: DFLA26078
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Neutral Protease (NP) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Substrate	Powder x 1	4°C
Stop Solution	12 mL x 1	4°C
Reaction Buffer	6 mL x 1	4°C
Dye Reagent	4 mL x 1	4°C, keep in dark
Standard	Powder x 1	4°C
Standard Diluent	5 mL x 1	4°C
Positive Control	Powder x 1	4°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.05 mmol/L - 5 mmol/L

General Description

Neutral Protease is frequently used as a secondary enzyme in conjunction with collagenase and/or other proteases in many primary cell isolation and tissue dissociation applications. Neutral Protease dissociates fibroblast-like cells more efficiently than epithelial-like cells, so it has also been used for differential isolation and culture applications. Other advantages are its non- mammalian (bacterial) source and its ability to be inhibited by EDTA. The assay is initiated with the enzymatic catalysis of casein by NP in neutral environment. The enzyme catalyzed reaction products can be measured at a colorimetric readout at 660 nm.

Citations