Native Staphylococcus aureus Protease Protein

Product Overview

Enzyme purified fromS. aureus, strain V8

Specificity

The enzymespecifically cleaves peptide bonds on the COOH terminal side of eitheraspartic or glutamic acids. In the presence of ammonium, the specificity islimited to glutamic sites.

Nature

Native

Tag/Conjugate

Unconjugated

Molecular Weight

27,700 (bysedimentation equilbrium).

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Monitoring of various protease activities has become a routine task for many biological laboratories. Our Amplite Universal Fluorimetric Protease Activity Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. The kits use fluorescent casein conjugates that are proven to be a generic substrate for a broad spectrum of proteases. In the intact substrate, casein is heavily labeled with a fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The kits provide all the essential components with an optimized mix and read protocol that can be easily automated to HTS instruments.

Keywords

Protease