Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Acidic Protease (ACP) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Substrate | Powder x 1 | 4°C |
| Stop Solution | 12 mL x 1 | 4°C |
| Reaction Buffer | 6 mL x 1 | 4°C |
| Dye Reagent | 4 mL x 1 | 4°C, keep in dark |
| Standard | Powder x 1 | 4°C |
| Standard Diluent | 5 mL x 1 | 4°C |
| Positive Control | Powder x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.05 mmol/L - 5 mmol/L
General Description
Acidic protease (ACP) can act on interior bond -C-NH- to hydrolyze the proteins of vegetable and animal in acid environment to form small peptides and amino acidic. The addition of this product to the processing of alcohol and distilled spirit can promote the growth of yeast to accelerate fermentation speed and improve alcohol productivity. In feedstuff industry, this product can be used as an additive by combined with other enzymes (such as cellulase, pectinase and β-glucanase, etc) for the preparation of complex feedstuff enzymes. It can further improve the nutrition value of feedstuff to accelerate the growth and increase the weight of livestock and poultry. The assay is initiated with the enzymatic catalysis of casein by ACP in acid environment. The enzyme catalyzed reaction products can be measured at a colorimetric readout at 660 nm.
Citations
Publication ()
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