Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Na+/K+ ATPase activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Substrate | Powder x 1 | -20°C |
| Activator | Powder x 1 | 4°C |
| Inhibitor | Powder x 1 | 4°C |
| Dye Reagent I | Powder x 1 | 4°C |
| Dye Reagent II | Powder x 1 | 4°C |
| Dye Reagent III | 20 mL x 1 | 4°C |
| Stop Solution | 4 mL x 1 | Room temperature |
| Standard ( umol/mL) | 5 | 1 mL x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.01 umol/mL - 5 umol/mL
General Description
Na+/K+ ATPase is widely distributed in plants, animals, microbes and cells, can catalyze the hydrolysis of ATP, ADP and inorganic phosphate. Na+/K+ ATPase catalyze the decomposition of ATP into ADP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. At the end of the reaction period, the dye reagent forms a color with released phosphate ion, which is measured on a plate reader 660 nm.
Citations
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