Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Ca2+/Mg2+ ATPase activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Substrate | Powder x 1 | -20°C |
| Activator | Powder x 1 | 4°C |
| Inhibitor | Powder x 1 | 4°C |
| Dye Reagent I | Powder x 1 | 4°C |
| Dye Reagent II | Powder x 1 | 4°C |
| Dye Reagent III | 20 mL x 1 | 4°C |
| Stop Solution | 4 mL x 1 | Room temperature |
| Standard ( umol/mL) | 5 | 1 mL x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.01 umol/mL - 5 umol/mL
General Description
Ca2+/Mg2+ ATPase is widely distributed in plants, animals, microbes, and cells, can catalyze the hydrolysis of ATP, ADP and inorganic phosphate. Ca2+/Mg2+ ATPase breaks down ATP to generate ADP and inorganic phosphate, ATP activity is determined by measuring the amount of inorganic phosphorus.
Citations
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