Sample
serum, plasma, cell culture medium
Detection Method
Sandwich ELISA
Intended Use
The mouse LBP kit has been developed for the quantitative measurement of natural and recombinant mouse LBP (both free and LPS-bound) in serum, plasma and culture medium.
Contents of Kit
1. Precoated ELISA modules, 1 plate
2. Detecting antibody (POD-labelled monoclonal antibody to mouse LBP) "Ready for use", 1 vial
3. Mouse LBP-standard, 1 vial
4. Mouse reference serum, 1 vial
5. PBS, 2 tab.
6. Dilution Buffer, 1 vial
7. Tween 20, 1 vial
8. Stopping solution, 1 vial
9. Substrate solution, 1 vial
Storage
Short time store at 2-8°C, Long time storage of vial 3 and 4 at -20°C or -80°C, Detecting monoclonal can be stored at 2-8°C.
Precision
Interassay variation coefficient: 7% till 13.6% depending of concentration
Intraassay variation coefficient: 2.4%, n=50 plasma samples
Detection Range
Effective range: 1-50 ng/mL
Citations
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