Lipoxygenase Assay Kit

Name: Lipoxygenase Assay Kit
SKU: DFLA26220
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Tissue extracts, Cell lysate, Cell culture media, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Lipoxygenase Activity in tissue extracts, cell lysate, cell culture media, other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Substrate I	Powder x 1	4°C
Substrate I Diluent	6 mL x 1	4°C
Substrate II	Powder x 1	4°C
Dye Reagent	1 mL x 1	4°C
Reaction Buffer	7 mL x 1	4°C
Standard ( mmol/L)	3	1 mL x 1	4°C
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.03 mmol/L - 3 mmol/L

General Description

Lipoxygenases (EC 1.13.11.-) are a family of (non-heme) iron-containing enzymes most of which catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4- pentadiene into cell signaling agents that serve diverse roles as autocrine signals that regulate the function of their parent cells, paracrine signals that regulate the function of nearby cells, and endocrine signals that regulate the function of distant cells. Lipoxygenase Microplate Assay Kit is based on oxidation of substrate to iodine. The intensity of the product color, measured at 470 nm, is proportional to the Lipoxygenase activity in the sample.

Citations