Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Glutathione S-transferase (GST) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 20 mL x 1 | 4°C |
| Substrate I | Powder x 1 | 4°C |
| Substrate I Diluent | 1 mL x 1 | 4°C |
| Substrate II | Powder x 1 | 4°C |
| Positive Control | Powder x 1 | 4°C |
| Instruction Manual | 1 | |
General Description
Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification. The Glutathione S-transferase Microplate Assay Kit is based upon the GST- catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro- 2,4-dinitrobenzene, which has the broadest range of isozyme detectability. Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.
Citations
Publication ()
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