Glutathione Peroxidase Assay Kit

Name: Glutathione Peroxidase Assay Kit
SKU: DFLA26138
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Other biological fluids, Tissue extracts, Cell lysate, Cell culture media

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Glutathione Peroxidase (GPX) activity in serum, plasma, tissue extracts, cell lysate, cell culture media, and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Substrate I	Powder x 1	4°C
Substrate II	1 mL x 1	4°C
Reaction Buffer	10 mL x 1	4°C
Stop Solution	15 mL x 1	4°C
Dye Reagent	Powder x 1	4°C
Standard	Powder x 1	4°C
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.01 mmol/L - 5 mmol/L

General Description

Glutathione Peroxidase (EC 1.11.1.9) family of enzymes play important roles in the protection of organisms from oxidative damage. GPX converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPX have been correlated with free radical related disorders. Glutathione Peroxidase Microplate Assay Kit is a sensitive assay for determining Glutathione Peroxidase activity in various samples. GPX reduces Hydroperoxide while oxidizing GSH to GSSG. GSH may react with DTNB. The decrease of GSH is proportional to GPX activity.

Citations