Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Glutamate content in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 8 mL x 1 | 4°C |
| Dye Reagent | Powder x 1 | 4°C, keep in dark |
| Diluent | 4 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C, keep in dark |
| Instruction Manual | 1 | |
Detection Range
0.01 mmol/L - 0.5 mmol/L
General Description
Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6- phosphate dehydrogenase, glutathione synthase and glutathione reductase. The enzyme catalyzed reaction products can be measured at a colorimetric readout at 412 nm.
Citations
Publication ()
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