Fumonisin ELISA kit

Sample

feed, cereal

Species Reactivity

N/A

Detection Method

cELISA

Intended Use

Fumonisin ELISA is a competitive enzyme immunoassay for the quantitative analysis of fumonisins in feed, cereal. The unique features of this kit are:
• High recovery (75-120%)
• Low detection limit
• A quick ELISA assay (less than one hour regardless of number of samples)

Contents of Kit

Fumonisin ELISA has the capacity for testing of 96 samples/controls. Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. The shelf life is at least 12 months when the kit is properly stored.
1. Fumonisin Antigen-coated Plate, 1 × 96-well Plate, 2-8°C
2. Fumonisin Standards: 2-8°C
Negative control, 0.8 mL
0.05 ng/mL, 0.8 mL
0.15 ng/mL, 0.8 mL
0.5 ng/mL, 0.8 mL
1.5 ng/mL, 0.8 mL
5 ng/mL, 0.8 mL
1000 ng/mL (spiking), 0.8 mL
3. Anti- Fumonisin Antibody, 14 mL. 2-8°C*
4. HRP-Conjugated Antibody, 0.2 mL. 2-8°C*
5. 10× Sample Extraction Buffer, 25 mL. 2-8°C
6. 20× Wash Solution, 28 mL. 2-8°C
7. Stop Solution, 12 mL. 2-8°C
8. TMB Substrate, 12 mL. 2-8°C
If you are not planning to use the kit for over 3 months, store Anti-Fumonisin Antibody and HRP-Conjugated Antibody at -20°C or in a freezer.

Sensitivity

Feed/Cereal 50

General Description

The fumonisins are a group of mycotoxins derived from Fusarium that grow on several agricultural commodities, mainly corn, in the field or during storage. More than ten chemical forms of fumonisins have been isolated, of which fumonisin B1 is the most prevalent in contaminated corn and is believed to be the most toxic. Fumonisin B1 is hepatotoxic and nephrotoxic in all animal species tested.
Fumonisins have also been reported to cause acute epidemics of disease in weanling or adult pigs, characterized by pulmonary edema and hydrothorax. Porcine pulmonary edema (PPE) is usually an acute, fatal disease and appears to be caused by pulmonary hypertension with transudation of fluids in the thorax, resulting in interstitial pulmonary edema and hydrothorax.
The Advisory exposure guidelines by the US FDA have recommended total dietary concentrations of fumonisins as follows: horses < 1 ppm, swine < 10 ppm, ruminants for slaughter and mink for pelt production < 30 ppm, poultry for slaughter < 50 ppm, breeding ruminant, mink, and breeding poultry < 15 ppm, and all other species and pets < 5 ppm. Regulatory guidelines for fumonisins are provided in most countries and the EU.