Anti-Spermidine polyclonal antibody

The aim of the present study was to produce goat embryo in different culture media through in vitro fertilization using cryopreserved black Bengal buck semen. Total 1265 fresh cumulus oocyte complexes (COGS) were collected by aspiration method with a 19 gauge hypodermic needle, washed 5-6 times and cultured in maturation media maintaining 5% CO, level at 38.5 degrees C with maximum humidity in a incubator. After 27 h of incubation cumulus cells were stripped off from matured oocytes and transferred to acidified Tyrode's medium for zona thinning and co-incubated with in vitro capacitated sperms for fertilization in Fert-BO media. In the experiment I, fresh buck semen and in experiment II, frozen buck semen was used for in vitro fertilization after in vitro processing. After 5 h of coincubation, presumptive zygotes were washed and co-incubated with oviductal cells in three different culture media (RVCL, mSOF, KSOM) for further development. In fresh group cleavage (%) were 37.76 +/- 2.98, 39.60 +/- 1.75, 29.01 +/- 1.74, and morula formation (%) were 7.72 +/- 3.38, 6.03 +/- 1.29 and 3.00 +/- 3.00 in RVCL, mSOF and KSOM media respectively. However, in frozen group cleavage (%) were 29.17 +/- 2.56, 27.70 +/- 2.31, 24.17 +/- 1.44 in RVCL, mSOF and KSOM media respectively and morula formation (%) was 2.93 +/- 0.97 only in RVCL media. These results indicate that cryopreserved black Bengal buck semen have competence to produce embryos and could be used for embryo development in RVCL media through in vitro fertilization.

An in vitro study was carried out to assess the addition of polyphenols in the chicken seminal plasma on sperm quality and antioxidant status at different storage periods. Twenty healthy breeding roosters of White Leghorn chicken having similar body weight were randomly selected. The polyphenols in the pomegranate peels were extracted using methanol as a solvent. Samples were split equally into three portions for inclusion of different concentrations of 0, 50 and 100 ppm of polyphenols, respectively. Estimation of catalase, inhibition of lipid peroxidation, reduced glutathione and superoxide dismutase was done at 0, 24 and 48 h intervals. The sperm motility invariably increased in all treatment groups (50, 100 ppm) than control in both storage conditions. Other physical attributes such as sperm concentration, live and dead spermatozoa did not differ over control regardless of concentrations and time periods of storage. Inhibition of lipid peroxide action was found significant at the end of 48 h of storage in both the polyphenol treatment groups than control, while there was no change at 0 and 24 h. Polyphenols from pomegranate peels improved spermatozoal motility and antioxidant activity in seminal plasma and it could be concluded that the polyphenols has capability to protect the spermatozoa against oxidative damage.