Animals were immunized with mu-calpain isolated from human erythrocytes [PubMed: 1426051]. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for the large subunit of human mu-calpain and shows no cross-reactivity with m-calpain. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Application Notes
It can be used for immunochemical and WB applications. It is a useful tool for detecting and localizing the large subunit of calpain.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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