C57BL/10 mouse spleen cells enriched for T lymphocytes
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Myeloma
NS-1
Clone
N2/81.26.22.6.IM
Cell Line Description
Animals were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody precipitates two polypeptides of 190000 and 105000 daltons, binds to human monocytes, polymorphonuclear leukocytes and a small population of lymphocytes. The antibody is capable of both natural killing and antibody dependent cellular cytotoxicity. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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