Animals were immunized with activated mouse macrophages. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts weakly with resident mouse peritoneal macrophages and strongly with mouse macrophages that have been activated by treatment with Macrophage Activating Factor (MAF) or thioglycollate. It does not react with rat macrophages nor with a panel of mouse macrophage, monocyte or lymphoid cell lines.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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