. It was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with transfected COS-7 cells expressing huIL-12R beta1.
Application Notes
Detection of huIL-12Rbeta1 by FC, WB and IP.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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