The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12. The antibody will immunoprecipitate IL-12. The 20C2 antibody does not bind the p40 or p35 subunits of IL-12. The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75. [38686]
Application Notes
Neutralizing and receptor binding assays; it will immunoprecipitate IL-12. The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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