Human IL 12 p40 ELISA Kit

Sample

Serum

Intended Use

Immunoenzymetric assay for measurement of human interleukin-12 (IL-12) in serum.

Storage

1. Before opening or reconstitution, all kits components are stable until the expiry date, indicated on the vial label, if kept at 2 to 8°C.
2. Unused strips must be stored, at 2-8°C, in a sealed bag containing a desiccant until expiration date.
3. After reconstitution, calibrators, controls and Specimen Diluent are stable for 4 days at 2 to 8°C. For longer storage periods,
aliquots should be made and kept at -20°C for maximum 2 months. Avoid successive freeze thaw cycles.
4. The concentrated Wash Solution is stable at room temperature until expiration date.
5. Freshly prepared Working Wash solution should be used on the same day.
6. After its first use, the conjugate is stable until expiry date, if kept in the original well-closed vial at 2 to 8°C.
7. The freshly prepared revelation solution is stable, before use, for maximum 15 minutes at room temperature and must be discarded afterwards.
8. Alterations in physical appearance of kit reagents may indicate instability or deterioration.

Sensitivity

The minimum detectable dose of IL 12 is < 2.0 pg/ml.

General Description

Human interleukin-12 (IL-12) is a 75 kDa lymphokine produced mainly by monocytes, macrophages, B-lymphocytes and dendritic cells. IL-12 shows an unusual heterodimeric structure composed of one 40 kDa (p40) and of one 35 kDa (p35) subunits linked together by disulfide bonds. p35 subunit is distantly related to IL-6 and G-CSF while p40 shows homology to the extracellular domain of the α chain of the IL-6 receptor. This suggests that IL-12 may have evolved from a cytokine/soluble receptor complex.
p40 is secreted in large excess over the biologically active heterodimer. p40 is involved in receptor binding but p35 is necessary
for signal transduction. Monomers and mainly homodimers of p40 show antagonist activity to IL-12.
In vivo, IL-12 appears to play a major role in auto-immune disease, in the resistance to bacterial and parasitic infections, in
antiviral responses including HIV, in the promotion of antitumor immunity. IL-12 has been shown to be a powerful adjuvent in
vaccination.