Anti-GST tag antibody

Silkworms are economically important insects that have the ability to produce large amounts of silk. They have mass breeding methods and silk glands, which are specialized tissues that secrete silk fibroin and sericin. Thus, the production of recombinant proteins in a transgenic silkworm system is a promising approach. We developed a silkworm, Bombyx mori, as a host expression insect for recombinant proteins and successfully produced different proteins including antibodies, glycoproteins, and membrane receptors. The thyroid hormone receptor (TR) is a regulatory factor for many physiological phenomena. It is a lipophilic protein that has DNA-binding and ligand-binding domains. Based on our previous experiences, it was inferred that the recombinant TR easily formed aggregates and precipitates which is potentially due to an unstructured hinge domain. We applied the silkworm expression system to produce mice TR beta 1 that was fused with glutathione 5-transferase. Using 160 larvae, the yield of the recombinant GST-TR beta was approximately 4 mg, and the purified GST-TR beta completely retained its physiological activity. Our results indicated that the recombinant TRP was secreted extracellularly using the silk fibroin signal peptide sequence. Moreover, we found that the expression system of silkworms was applicable to nuclear proteins.

Copper oxide nanoparticles are used in various fields. The increased use also enhances the release of these particles into the aquatic environment. CuO-NPs cause toxicity to every stage of aquatic organisms' lives. This study investigates the effects of CuO-NPs on the antioxidant system of rohu (Labeo rohita). Four treatment groups were maintained in this study including; T-0 (control), T-1 (0.5 mu g/L), T-2 (1 m mu/L) and T-3 (1.5 m mu/L) CuO-NPs for 45 days. 25% of water was exchanged on daily basis with redosing with CuO-NPs. The samples were taken for antioxidant analysis after 45 days. The samples of gill, heart, liver and kidney were analyzed with graphite furnace atomic absorption spectroscopy to measure the absorbed concentration of Cu. Results showed an increase in Cu accumulation in tissues (P <= 0.001) in all the treatments compared to control. The accumulation pattern of Cu was gill > kidney > liver > heart. Besides, the treatment also disturbs the GST level in the liver, heart, kidney and gill tissue. A sharp decline in GST activities (P <= 0.05) was observed due to raised in MDA content. The high GSH level (P <= 0.001) in liver indicates an alteration in the defensive mechanism against. This study concluded that Cu has more potential to deposit in soft tissues from CuO-NPs and cause disruption in antioxidant defenses by raising the glutathione (GSH) level. This study recommended taking preventive measures for minimizing the entry of CuO-NPs into the aquatic environment and building up in the food chain.