Anti-Acetyl-Lysine (C-terminal) polyclonal antibody

Name: Anti-Acetyl-Lysine (C-terminal) polyclonal antibody
SKU: DPATB-H81026
Rating: 5 (1 reviews)

Sheep anti-Human Acetyl-Lysine (C-terminal) polyclonal antibody for ICC, IF, WB, ELISA

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Tetra-acetylated synthetic N-terminal peptide, corresponding to amino acids 1-18 of Human histone H4.

Conjugate

Unconjugated

Target

Alternative Names

Acetylation of lysine is an important, reversible posttranslational modification. Lysine acetylation is a dynamic process that is used to regulate protein-DNA and protein-protein interactions. Lysine acetylation involves the reversible transfer of acetylC

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Use of a phosphopeptide as a ligand to purify phospholipase A(2) from the venom of Crotalus durisuss terrificus by affinity chromatography

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Authors: Saavedra, Soledad L.; Acosta, Gerardo; Avila, Lucia; Giudicessi, Silvana L.; Camperi, Silvia A.; Albericio, Fernando; Cascone, Osvaldo; Martinez Ceron, Maria C.

Abstract

The venom of Crotalus durissus terrificus (Cdt) is a source of a wide variety of toxins, some of them with interesting pharmacological applications. Of these toxins, the phospholipase A(2) (PLA(2)) subunit of crotoxin (Ctx) has been studied for its potential as an antiviral and antibacterial agent. Peptides have proven useful ligands for the purification of numerous molecules, including antibodies, toxins, enzymes and other proteins. Here, we sought to use a phosphopeptide (P-Lys) as a ligand for PLA(2) purification. P-Lys was synthesized in solid phase on Rink-Amide-ChemMatrix resin, immobilized on NHS-agarose, and then evaluated as a chromatographic matrix. Under the best conditions, total protein adsorption reached 39% and only the eluate fraction presented PLA(2) activity. Analysis of the eluate by SDS-PAGE showed three bands, one corresponding to the molecular weight of PLA(2) (14 kDa). Said bands were analyzed by mass spectrometry and identified as PLA(2) and its multimers. The final product showed a purity of over 90%. In addition, slightly changing the process conditions also allowed the isolation of crotamine.

Selective Cyclized alpha-Melanocyte-Stimulating Hormone Derivative with Multiple N-Methylations for Melanoma Imaging with Positron Emission Tomography

ACS OMEGA

Authors: Zhang, Chengcheng; Zhang, Zhengxing; Zeisler, Jutta; Colpo, Nadine; Lin, Kuo-Shyan; Benard, Francois

Abstract

In this study, we designed and evaluated a novel alpha-melanocyte-stimulating hormone derivative with four N-methylations for melanocortin 1 receptor-targeted melanoma imaging with positron emission tomography (PET). The resulting peptide, DOTA-Pip-Nle(4)-Cyclo [Asp(5)-N-Me-His(6)-D-Phe(7)-N-Me -Arg(8)-N-Me-Trp(9)-N-Me-Lys(10)]alpha MSH4-10-NH2 (CCZ01099), showed high receptor selectivity, greatly improved stability, and rapid internalization. [Ga-68]Ga-CCZ01099 showed clear tumor visualization and excellent tumor-to-normal tissue contrast with PET imaging in a preclinical melanoma model. Therefore, CCZ01099 is a promising compound for imaging and potentially radioligand therapy for melanoma.