Mouse Anti-Acetylated Lysine Hybridoma [8G9] (CSC-LS01)

Regulatory status: For research use only, not for use in diagnostic procedures.

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General Information


Immunogen
Acetylated keyhole limpet hemocyanin (KLH) was used as immunogen. The cell line was screened against non acetylated KLH as a control in addition to BSA and histones.
Isotype
IgG1
Fusion Species
Mouse X Mouse Hybridoma
Myeloma
SP2/0 Mus musculus
Clone
8G9
Cell Line Description
2 x 10^6 – 2 x 10^7 cells/ml in freezing media. 10% dimethylsulfoxide (DMSO) and 90% Fetal Bovine Serum (FBS). Product is frozen.
Pan Specific. This antibody will detect proteins containing acetylated lysine residues in ELISA and Western Blots. Bovine albumin and Avian histones were tested.
Format
Frozen
Morphology
lymphoblast
Growth Properties
suspension

Culture Method


Complete Growth Medium
DMEM +FBS
Storage
Store in liquid nitrogen.

Target


Introduction
Acetylation of lysine is an important, reversible posttranslational modification. Lysine acetylation is a dynamic process that is used to regulate protein-DNA and protein-protein interactions. Lysine acetylation involves the reversible transfer of acetylCoA to the ε- amino group of lysine, which neutralizes its positive charge.

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