Synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
313-22B9
Cell Line Description
Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the antibody recognizes proteins of 140, 000, 70, 000, 60, 000 and 55, 000 daltons. The IgG1 reactivity of the antibody with the src peptide (276-287) was confirmed by ELISA.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to fetal bovine serum, add 10% (v/v) DMSO
Citations
Publication ()
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