Phospho-PKR Thr451 ELISA Kit

Sample

cell lysate

Species Reactivity

Human

Intended Use

The Phospho-PKR Thr451 ELISA Kit is designed to detect and quantify the level of human PKR protein phosphorylated at threonine 451 (phospho-PKR Thr451). Since the amino acid sequence surrounding threonine 451 is not conserved in mouse and rat PKR, this ELISA kit can not be used for mouse and rat cells.
This assay is intended for the measurement of phospho-PKR Thr451 in lysates from human cells.
This assay kit is for research use only and not for use in diagnostic or therapeutic procedures.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Sensitivity

The limit of detection (defined as such a concentration of phospho-PKR Thr451 giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 0.505 units/mL of sample.

General Description

The double-stranded RNA-activated protein kinase, PKR, is a ubiquitously expressed serine/threonine protein kinase that plays a key role in the innate immunity response to viral infection in higher eukaryotes and has also been implicated in several cellular signal transduction pathways. The dsRNAs-mediated activation leads to autophosphorylation of PKR and allows the kinase to phosphorylate its natural substrate, the α subunit of initiation factor eIF2, resulting in rapid inhibition of translation and suppression of virus spread. PKR also has been implicated in regulating other cellular functions such as differentiation, transcription, signal transduction, cell growth and apoptosis in the event of virus infection and other forms of cellular stress. It was reported that the activation of PKR in adipose and liver tissue is caused by obesity. In the absence of PKR, metabolic deterioration due to excess energy or nutrition is alleviated. These findings demonstrate that PKR is an important component of inflammation complex that responds to nutrients and organelle dysfunction.
The phosphorylation status of PKR at threonine 451 is shown to be a nice marker of the activation of PKR in vitro as well as in vivo.