PKR (Phospho-Thr446) ELISA Kit

Name: PKR (Phospho-Thr446) ELISA Kit
SKU: DEIA-XYA1477
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Cultured cells

Detection Method

Indirect-ELISA

Intended Use

The PKR (Phospho-Thr446) ELISA Kit is a convenient, lysate- free,high throughput and sensitive assay kit that can monitor target protein Phosphorylation and expression profle in cells. The kit can be used for measuring the relative amounts of phosphorylated proteins in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PKR (Phospho-Thr446).

Contents of Kit

No.	Components	Size	Storage Conditions
1	96-Well Cell Culture Clear-Bottom Microplate	2 Plates	4°C
2	10× TBS	30 ml	4°C
3	Quenching Buffer	24 ml	4°C
4	Blocking Buffer	50 ml	4°C
5	15× Wash Buffer	60 ml	4°C
6	100× Anti-PKR (Phospho-Thr446) Antibody	60 μl	4°C
7	100× Anti-PKR Antibody	60 μl	4°C
8	100× Anti-GAPDH Antibody	60 μl	4°C
9	HRP-Conjugated Anti-Rabbit IgG Antibody	12 ml	4°C
10	HRP-Conjugated Anti-Mouse IgG Antibody	12 ml	4°C
11	Primary Antibody Diluent	12 ml	4°C
12	Ready-to-Use Substrate	12 ml	4°C
13	Stop Solution	12 ml	4°C
14	Crystal Violet Solution	12 ml	4°C
15	SDS Solution	24 ml	4°C
16	Adhesive Plate Sealers	2 Seals