Pig C-Peptide ELISA Kit

Sample

Serum, plasma, tissue homogenates

Species Reactivity

Pig

Detection Method

sELISA

Intended Use

For the quantitative determination of pig C-peptide concentrations in serum, plasma, tissue homogenates.

Contents of Kit

1. Assay plate (12×8 coated Microwells): 1(96 wells)
2. Standard (Freeze dried): 2
3. Biotin-antibody (100× concentrate): 1×120 μl
4. HRP-avidin (100× concentrate): 1×120 μl
5. Biotin-antibody Diluent: 1×15 ml
6. HRP-avidin Diluent: 1×15 ml
7. Sample Diluent 1×50 ml
8. Wash Buffer (25× concentrate): 1×20 ml
9. TMB Substrate: 1×10 ml
10. Stop Solution 1×10 ml
11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1

Storage

Unopened kit can be stored at 2 - 8°C until the expiration date. Do not use the kit beyond the expiration date.
Opened kit:
1. Coated assay plate may be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
2. Standard may be stored for up to 1 month at 2 - 8° C. If don't make recent use, better keep it store at -20°C.
3. Biotin-antibody may be stored for up to 1 month at 2 - 8° C. If don't make recent use, better keep it store at -20°C.
4. HRP-avidin may be stored for up to 1 month at 2 - 8° C. If don't make recent use, better keep it store at -20°C.
5. Other reagents may be stored for up to 1 month at 2 - 8°C.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

1.25 ng/ml-80 ng/ml.

Sensitivity

0.25 ng/mL