C-Peptide ELISA Kit

Sample

serum, plasma, culture supernatants, cell lysates

Species Reactivity

Human

Contents of Kit

1. 96-well plate coated with secondary antibody (Item A)
2. 20x Wash Buffer (Item B)
3. EIA C-Peptide standard (Item C)
4. Anti-C-Peptide Detection Antibody (Item N)
5. EIA Assay Diluent A (Item D)
6. EIA 5x Assay Diluent B (Item E)
7. Biotinylated C-Peptide (Item F)
8. 200x HRP-streptavidin (Item G)
9. C-Peptide Positive Control Sample, Lyophilized (Item M)
10. TMB Substrate solution (Item H)
11. Stop Solution (Item I)

Storage

Store the kit at -20°C. It remains active for up to 1 year. Avoid repeated freeze-thaw cycles.
The reconstituted standard should be stored at -20°C or -70°C (-70°C is recommended). Opened microplate strips or reagents may be store for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Sensitivity

772 pg/mL

General Description

The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide based on the principle of competitive enzyme immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti-C-Peptide antibody, both biotinylated C-Peptide, and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide then interacts with Streptavidinhorseradish peroxidase (SA-HRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide can be established and the concentration of C-Peptide in the samples can be calculated accordingly.