Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Mouse Tnnt1 (Troponin T, slow skeletal muscle) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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WARREN, KS; LIN, JJC; et al. FORCED EXPRESSION AND ASSEMBLY OF RAT CARDIAC TROPONIN-T ISOFORMS IN CULTURED MUSCLE AND NONMUSCLE CELLS. JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY 14:619-632(1993).
Andersson, H; Kagedal, B; et al. Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing. ANALYTICAL AND BIOANALYTICAL CHEMISTRY 398:1395-1402(2010).
COA
Certificate of Analysis
